Planar Functionalized Surfaces for Direct Immunoaffinity Desorption/Ionization Mass Spectrometry

Planar Functionalized Surfaces for Direct Immunoaffinity Desorption/Ionization Mass Spectrometry.

BACKGROUND Recent studies show that the haptoglobin phenotype in individuals with diabetes mellitus is an important factor for predicting the risk of myocardial infarction, cardiovascular death, and stroke. Current methods for haptoglobin phenotyping include PCR and gel electrophoresis. A need exists for a reliable method for high-throughput clinical applications. Mass spectrometry (MS) can in ...

Functionalized nanoparticles and nanostructured surfaces for surface-assisted laser desorption/ionization mass spectrometry.

Surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF-MS) using nanoparticles (NPs) and nanostructured surfaces as the LDI-assisting nanomaterials is a soft ionization technique that features minimal fragmentation of analytes. As compared to traditional matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) using organic ma...

Immunoaffinity Enrichment and Mass Spectrometry Analysis of Protein Methylation

Protein methylation is a common posttranslational modification that mostly occurs on arginine and lysine residues. Arginine methylation has been reported to regulate RNA processing, gene transcription, DNA damage repair, protein translocation, and signal transduction. Lysine methylation is best known to regulate histone function and is involved in epigenetic regulation of gene transcription. To...

Rapid, direct enzyme immunoassay of 11-keto-thromboxane B2 in urine, validated by immunoaffinity/gas chromatography-mass spectrometry.

We have developed a direct enzyme immunoassay (EIA) for quantifying immunoreactive 11-keto-thromboxane B2 (iKTXB) in unprocessed human urine. Cross-reactivity with other thromboxane metabolites and prostanoids was negligible. Analytical recovery of 11-keto-TXB2 in urine specimens was 97.4% to 99.8%. Total imprecision for two clinical specimens was 8.5% and 12.2%. Intake of acetylsalicylic acid ...

Mass spectrometry of polymers and polymer surfaces.